Figure 1. Experimental design and testing procedures of the study. Four conditions were based on the rest between three Loughborough Intermittent Shuttle Tests (LIST = indicated by ); one (1d), two (2d), three (3d) and 4 days (4d) interval rest between three Loughborough Intermittent Shuttle Tests performed consecutively by 13 participants, respectively. Time course of the measurements are indicated by X for each condition; B: baseline, one day before the first LIST, Day 1-14: days from the first LIST. The measurements in the LIST days were taken 1 hour after the LIST. All measurements were taken at 1, 2, 3, 4 and 5 days after the last LIST for all conditions. Muscle damage measurements were also taken between the first and second, and second and third LIST; days 2 and 4 for the 2d, days 2, 3, 4, and 5 for the 3d condition, and days 2, 3, 4, 6, 7, and 8 for the 4d conditions. The measurements included maximal voluntary isometric contraction torque of the knee extensors and flexors, muscle soreness, countermovement jump, 30-m dash, 30-m timed hop, agility T-test, 6 x 10-m shuttle run and Yo-Yo intermittent recovery test level 1 test, plasma creatine kinase activity, myoglobin concentration, glutamic oxaloacetate transaminase and glutamic pyruvic transaminase activity, uric acid, potassium phosphorus, high sensitivity cardiac troponin T concentrations and osmolality.