Figure 1. Skin surface SIgA and HBD-2 collection (a). First, the open end of a polypropylene tube was placed on the skin surface. Second, 1 mL of extraction liquid was poured into the tube. Finally, the liquid was stirred at 9,000 rpm with a microtube homogenizer for 60 s and collected. Skin-SIgA, HBD-2, staphylococci and moisture content of the stratum corneum measurement sites (b). Skin-SIgA and HBD-2 were collected from Ch1 and Fa1 at 18:30 (Before exercise; pre), Ch2 and Fa2 at 19:30 (After exercise; post), Ch3 and Fa3 at 20:30 (After 60 min of exercise; 60 min), and Ch4 and Fa4 at 21:30 (After 120 min of exercise; 120 min). Staphylococci were harvested from Ch5 and Fa5 at 18:30 (pre), Ch6 and Fa6 at 19:30 (post), Ch7 and Fa7 at 20:30 (60 min), and Ch8 and Fa8 at 21:30 (120 min).