IGF-1 Gene Expression in Rat Colonic Mucosa After Different Exercise Volumes

Table 1. Primer sequences used in quantitative real time RT-PCR.

GenBank Acc. No.	Gene name	PS forward 5´ to 3´	PS reverse 5´ to 3´	Amplicon size (bp)	AT	QT
NM 001101	Î²-actin	CTA CGT CGC CCT GGA CTT CGA GC	GAT GGA GCC GCC GAT CCA CAC GG	385	60°C	79°C
NM 012495	AldolaseA	ATGCCCCACCCATACCCAGCACT	AGCAGCAGTTGGCGGTAGAAGCG	191	64°C	79°C
NM 002046	GAPDH	GTC TTC ACC ACC ATG GAG AAG G	TCA TGG ATG ACC TTG GCC AG	198	60°C	79°C
NM 001082477	IGF-1	AAG CCT ACA AAG TCA GCT CG	GGT CTT GTT TCC TGC ACT TC	166	58°C	79°C
NM 052807	IGF-1R	AAA ACC ATC GAT TCT GTG ACG	GGT TCT TCA GGA AGG ACA AGG	199	60°C	79°C
NM 012588	IGF-BP3	CGC TAC AAA GTT GAC TAT GAG	CGT CTT TCC CCT TGG T	292	60°C	79°C

Abbreviations: GenBank Acc. No.= GenBank Accession number, PS = primer sequence, AT = annealing temperature, QT = quantification temperature, bp = base pairs, GAPDH = glycerine aldehyde 3-phosphate dehydrogenase, IGF-1 = insulin-like growth factor-1, IGF-1R = IGF-1 receptor, IGF-BP3 = IGF-binding protein 3.