Table 1. Blood perfusion, biomechanical properties, pressure pain threshold and reactive strength index (muscle power) of the gastrocnemius muscle in the experimental and sham group during Rest and Post-DN period (1-5 min and 24 h).

Variables	groups	Rest	Post 1-5 minutes	Post 24 hours	ES ηp 2	Groups effect	Time effect	time × group effect
PU	eDN	7.91 ± 0.73	12.41 ± 0.93 ‡c	9.39 ± 1.37 † b	0.685	65.054 <.001	39.01 <.001	39.198 <.001
	qDN	7.58 ± 0.65	7.62 ± 0.83	7.8 ± 0.8
T [Hz]	eDN	18.15 ± 1.87	16.13 ± 1.52 ‡a	14.77 ± 1.09 ‡c	0.583	12.991 .002	23.446 <.001	25.149 <.001
	qDN	19.42 ± 2.51	19.45 ± 2.66	19.48 ± 2.05
S [N/m]	eDN	326.6 ± 57.75	303.4 ± 54.79*	280.8 ± 47.61 ‡	0.365	3.083 .096	13.088 <.001	10.356 <.001
	qDN	354.7 ± 78.49	355.2 ± 77.04	352 ± 69.9
E	eDN	0.96 ± 0.1	0.91 ± 0.13	0.84 ± 0.1 ‡a	0.465	5.052 .037	3.632 0.037	15.649 <.001
	qDN	0.97 ± 0.08	1.01 ± 0.08	1.01 ± 0.11
PPT [N/cm]	eDN	112.94 ± 5.93	128.52 ± 5.67 ‡c	136.15 ± 8.24 ‡c	0.788	28.168 <.001	58.631 <.001	67.073 <.001
	qDN	107.33 ± 11.16	104.25 ± 7.95	107.19 ± 11.50
RSI [m/s]	eDN	2.10 ± 0.21	2.24 ± 0.24*	2.35 ± 0.15 ‡b	0.414	10.323 .005	5.924 .006	11.659 <.001
	qDN	1.98 ± 0.2	1.92 ± 0.24	1.94 ± 0.22

eDN – experimental group; qDN – quasi group; PU – perfusion unit; T- uscle tone; S – stiffness; E – elasticity; PPT - pressure pain threshold; RSI - reactive strength index.*, † , ‡: significant difference between Post 1-5min- or 24h vs. Rest measures (p < .05; p < .01; p < .001 – respectively);a, b, c: significant difference between groups (p < .05; p < .01; p < .001 – respectively).