In boys, muscle power and strength fluctuate with time-of-day with morning nadirs and afternoon maximum values. However, the exact underlying mechanisms of this daily variation are not studied yet. Thus, the purpose of this study was to examine the time-of-day effects on electromyographic (EMG) parameters changes during a Wingate test in boys. Twenty-two boys performed a 30-s Wingate test (measurement of muscle power and fatigue) at 07:00 and 17:00-h on separate days. Surface EMG activity was recorded in the Vastus lateralis, rectus femoris and vastus medialis muscles throughout the test and analyzed over a 5-s span. The root-mean-square (RMS) and mean-power-frequency (MPF) were calculated. Neuromuscular efficiency (NME) was estimated from the ratio of power to RMS. Muscle power (8.22 ± 0.92 vs. 8.75 ± 0.99 W·kg^-1 for peak power and 6.96 ± 0. 72 vs. 7.31 ± 0.77 W·kg^-1 for mean power, p < 0.001) and fatigue (30.27 ± 7.98 vs. 34.5 ± 10. 15 %, p < 0.05) during the Wingate test increased significantly from morning to evening. Likewise, MPF (102.14 ± 18.15 vs. 92.38 ± 12.39 Hz during the first 5-s, p < 0.001) and NME (4.78 ± 1.7 vs. 3.88 ± 0.79 W·mV^-1 during the first 5-s, p < 0.001) were higher in the evening than the morning; but no significant time-of-day effect was noticed for RMS. Taken together, these results suggest that peripheral mechanisms are more likely the cause of the child’s diurnal variations of muscle power and fatigue during the Wingate test.