The Bioharness^™ monitoring system may provide physiological information on human performance but there is limited information on its validity. The objective of this study was to assess the validity of all 5 Bioharness^™ variables using a laboratory based treadmill protocol. 22 healthy males participated. Heart rate (HR), Breathing Frequency (BF) and Accelerometry (ACC) precision were assessed during a discontinuous incremental (0-12 km·h^-1) treadmill protocol. Infra-red skin temperature (ST) was assessed during a 45 min^-1 sub-maximal cycle ergometer test, completed twice, with environmental temperature controlled at 20 ± 0.1 °C and 30 ± 0.1 °C. Posture (P) was assessed using a tilt table moved through 160°. Adopted precision of measurement devices were; HR: Polar T31 (Polar Electro), BF: Spirometer (Cortex Metalyser), ACC: Oxygen expenditure (Cortex Metalyser), ST: Skin thermistors (Grant Instruments), P:Goniometer (Leighton Flexometer). Strong relationships (r = .89 to .99, p < 0.01) were reported for HR, BF, ACC and P. Limits of agreement identified differences in HR (-3.05 ± 32.20 b·min^-1), BF (-3.46 ± 43.70 br·min^-1) and P (0.20 ± 2.62°). ST established a moderate relationships (-0.61 ± 1.98 °C; r = 0.76, p < 0.01). Higher velocities on the treadmill decreased the precision of measurement, especially HR and BF. Global results suggest that the BioharressTM is a valid multivariable monitoring device within the laboratory environment.