Pre-practice euhydration is key in the prevention of heat related injuries. The pre-practice hydration status of male National Collegiate Athletic Association (NCAA)-Division III athletes and the effects of a direct hydration regimen have yet to be investigated therefore; the aim of the study was 1) to analyze the pre-practice hydration status of current NCAA-DIII male athletes and 2) assess the impact of a directed intervention on pre-practice hydration status. The study was divided into baseline, pre and post intervention phases. For baseline, hydration status through urine specific gravity (USG) and anthropometric indices were measured prior to morning practice. Following baseline, pre-intervention commenced and participants were assigned to either control (CON) or experimental (EXP) groups. The CON and EXP group participants were instructed to maintain normal hydration and diet schedules and record fluid intake for seven days leading to post-intervention. The EXP group participants were asked to consume an additional 23.9 fl oz (~ 750 ml) per day for one week (7 days) leading to post-intervention. After 7 days the same measures were taken. At baseline, the majority of the participants were hypohydrated. Following the intervention, the EXP group participants consumed significantly more fluids than the participants in the CON group (3277.91 ± 1360. 23 ml vs 1931.54 ± 881.81 ml; p < 0.05). A-two-way repeated measure ANOVA revealed a non-significant time or treatment effect for USG or body mass but did demonstrate a significant USG interaction. In addition, an independent t-test examining absolute changes in USG demonstrated a significant difference between groups in which the EXP group improved hydration status and the CON group did not (-0.02 ± 0.006 vs 0.001 ± 0.005 ml; p < 0.05). In addition, there was no significant (p >0.05) difference in the regression slopes or intercepts between the CON and EXP groups when expressed as daily fluid intake per kg body (ml·kg^-1) and change in USG from pre-intervention to post-intervention. Most of the participants were hypohydrated at baseline/pre-intervention and the direct hydration intervention improved post-intervention hydration status but only to a small extent.