Too high demands, combined with a lack of resources, are often detrimental to athletic health and well-being. However, a valid and reliable instrument to investigate different dimensions of demands and resources in sport is currently unavailable. Therefore, the present study examines the psychometric properties of an existing and well-validated survey instrument on demands and resources at task-level that was adapted to sport. This instrument, the Demand-Induced Strain Compensation Questionnaire for Sport (DISQ-SPORT), was empirically tested among 1,101 athletes (416 females and 685 males) from a variety of sports and in different languages. Results supported the proposed six-factor structure of the instrument, consisting of physical, cognitive, and emotional demands and resources. Internal consistencies of all subscales were satisfactory and the instrument was invariant across type of sport, competitive level and language. Continued evaluation of the psychometric properties of the DISQ-SPORT, especially in terms of content validity and test-retest stability, is nevertheless warranted. Theoretical and practical implications as well as areas for future research are discussed.