The aims of this study were to: (1) analyze and compare the stroke kinematics between junior and senior elite male swimmers in every section of the race during the 50-m freestyle event, and; (2) identify stroke frequency (SF)–stroke length (SL) combinations on swim speed independently for junior and senior swimmers in each section of the 50-m freestyle event. Eighty-six junior swimmers (2019) and 95 seniors (2021) competing in the 50-m long course meter LEN Championships were analyzed. The t-test independent samples (p ≤ 0.05) were used to compare juniors and seniors. The SF and SL combinations on swim speed were explored using three-way ANOVAs. Senior swimmers were significantly faster in the 50-m race than juniors (p < 0.001). Speed presented the largest significant difference (p < 0.001) in section S0-15 m (start until the 15^th meter mark) being seniors fastest. Both junior and senior swimmers revealed a significant categorization (p < 0.001) by stroke length and stroke frequency in each race section. It was possible to model several SF–SL combinations for seniors and juniors in each section. The fastest swim speed in each section, for seniors and juniors independently, was achieved by a SF–SL combination that may not be the fastest SF or the longest SL. Coaches and swimmers must be aware that despite the 50-m event being an all-out bout, several SF–SL combinations were observed (independently for juniors and seniors), and they differ between race sections.