This study investigated the impact of a 4-week dry-land short sprint interval program (sSIT) on a swim ergometer, when incorporated into long aerobic-dominant in-water swimming training, on the physiological parameters, hormonal factors, and swimming performance of well-trained swimmers. Sixteen participants (age = 25 ± 6 years, height = 183 ± 6 cm, weight 78 ± 6 kg, body fat = 10.6 ± 3.1%) were randomized to either a long aerobic-dominant in-pool training plus three sessions/week of sSIT or a control group (CON) who didn’t engage in SIT. sSIT consisted of 3 sets of 10 × 4 s, 10 × 6 s, and 10 × 8 s all-out sprints interspersed by 15, 60, and 40 s recovery between each sprint, respectively. Pre- and post-training assessments included peak oxygen uptake (V̇O₂peak), O₂pulse (V̇O₂/HR), ventilation at V̇O₂peak (V̇_E@V̇O₂peak), peak and average power output, and freestyle swim performance at 50, 100, and 200-m distances, stroke rate, as well as testosterone and cortisol. sSIT resulted in significant improvements in V̇O₂peak (5.8%), O₂pulse (4.7%), V̇_E@V̇O₂peak (7.1%), peak and average power output (6.7% and 13.8%, respectively), total testosterone (20%), testosterone to cortisol ratio (16.1%), and 50, 100, and 200-m freestyle swimming performance (-2.2%, -1.2%, and -1.1%, respectively). Furthermore, the observed alterations in the physiological, biochemical, and performance adaptations were significantly more substantial in the sSIT group than the CON group (p ≤ 0.05), demonstrating no modifications during the 4-week long aerobic-dominant in-water swimming without sSIT. The current research effectively established that supplementing standard long aerobic-dominant in-water swim training with three weekly dry-land sSIT sessions triggers adaptive mechanisms that foster enhancements in the aerobic and anaerobic capacity and swimming performance in well-trained swimmers.