This preliminary study aimed to investigate the effects of a six-week low-frequency electromyostimulation training (10Hz) on the cardiovascular, respiratory and muscular systems. To that purpose, aerobic capacity, knee extensor muscles strength and architecture, muscle sympathetic nervous activity, blood pressure and heart rate have been evaluated in one healthy male subject (33 year-old, 1.73 m, 73 kg). Results showed improvement of aerobic capacity (+4.5% and +11.5% for maximal oxygen uptake and ventilatory threshold) and muscle strength (+11% and +16% for voluntary and evoked force). Moreover, for the first time, this study demonstrated low-frequency training effects on muscle architecture (+3%, +12% and -11% for muscle thickness, pennation angle and fascicle length) and cardiovascular parameters (-22%, -18% and -21% for resting muscle sympathetic nervous activity, heart rate and mean blood pressure). Interestingly, these results suggest that this method may have beneficial effects on all systems of the body. The investigation of training effects on muscle architecture and cardiovascular parameters should therefore be pursued since highly deconditioned subjects are likely to fully benefit from these adaptations.