This is the first study designed to examine differences in BLA, HR, and RPE responses during exercise with three different variations of circuit weight-training protocols (TRAD, ACWT, CWIT) in recreationally active women. The overall findings supported the hypothesis that including high-intensity sprint bouts into the weight-training protocol (i.e., CWIT) would elicit increased measures of BLA, HR, and RPE when compared to the TRAD and ACWT protocols. Previous researchers have noted increased BLA concentrations during TRAD workouts when compared to controls and more traditional weightlifting protocols (Garbutt et al., 1994; Marx, et al., 2001). To our knowledge, no researchers have examined the acute BLA response to ACWT and CWIT protocols. In the current study, both TRAD and ACWT resulted in similar BLA levels until EX C3, at which point the ACWT protocol produced higher concentrations. The CWIT protocol resulted in higher BLA levels at all exercise time intervals throughout the workout compared to TRAD and ACWT. Measuring BLA provides researchers with a way to evaluate the immediate physiological effects of an exercise protocol (Brooks et al., 2000). As indicated by the higher BLA levels, CWIT was found to be a higher intensity exercise when compared to the more customary forms of TRAD previously investigated (Garbutt et al., 1994). Traditional circuit training (TRAD) has been suggested as a way to increase excess post-exercise oxygen consumption or EPOC (Lavinas Da Silva et al., 2010). Since EPOC is directly related to the exercise intensity, it is possible that CWIT may elicit a higher EPOC than TRAD. High BLA levels, such as those observed with CWIT, are indicative of the accumulation of lactate and anaerobic energy production, and possibly higher EPOC. The increased BLA concentrations found in the combined circuit weight-interval training (CWIT) protocol are evidence of possibly greater energy expenditure for this workout, when compared to TRAD (CWIT BLA 132% > TRAD BLA) and ACWT (CWIT BLA 60% > ACWT BLA) protocols of similar duration. The possibility of a single exercise session of CWIT allowing for greater energy expenditure than a single session of TRAD or ACWT is noteworthy. Increasing energy expenditure in an exercise session on a regular basis will result in an improved fitness level over a shorter time period. Though no method currently exists to directly measure caloric consumption based upon lactate production, a strong possibility remains that the often-ignored anaerobic metabolism contributes a significant portion of total body energy expenditure. Furthermore, the inclusion of high-intensity interval bouts in this type of exercise program is potentially advantageous for those attempting to lose weight, specifically body fat (Schoenfeld and Dawes, 2009). The higher BLA concentrations found in CWIT may have occurred as a result of greater recruitment of Type II fast twitch muscle fibers. Fast twitch fibers produce greater amounts of lactate than slow twitch fibers, even when oxygen is present (Brooks et al., 2000). The substitution of sprint bouts for rest or moderately paced aerobic bouts in between mini-circuits may have stimulated greater fast twitch muscle fiber recruitment during the CWIT protocol, as evidenced by the higher BLA concentrations. Consequently, greater stimulation is possible for muscles and systems that are not otherwise adequately overloaded with more traditional forms of circuit weight training. Exercise variables, such as frequency, intensity, and duration, must be manipulated in order to create an overload with which the body is unfamiliar and to achieve a subsequent training effect (Pollock et al., 1998). Heart rate is frequently used as a guide to set exercise intensity due to the relatively linear relationship between HR and VO2, and HR and exercise intensity (Brooks et al., 2000; Whaley, 2006). Manipulation of intensity might be one factor differentiating the TRAD, ACWT, and CWIT protocols in the present study. All three workouts were of similar duration (i.e., 40 minutes), but only CWIT resulted in higher HR values at all exercise times compared to those of TRAD and ACWT (CWIT HR 14% > ACWT HR and TRAD HR). It is possible that individuals participating in CWIT were working at a higher intensity throughout the entire session, thereby creating an environment that could stimulate greater cardiovascular adaptations with regular use of the CWIT protocol. Caution should be employed when using HR alone in an attempt to assess the intensity of resistance exercise (Petersen et al., 1988). As noted by Gotshalk et al., 2004, traditional circuit weight training (TRAD) produced HR values within the recommended range for developing cardiovascular fitness; however, oxygen consumption does not follow the same linear path in such training. Increased HR is likely due to the inclusion of small muscle group exercises and may be caused by a greater static muscular load that occurs with TRAD, which is not seen in rhythmic aerobic exercise (Garbutt and Cable, 1998). While positive cardiovascular adaptations are indeed possible with higher intensities of exercise, HR should be interpreted carefully when resistance training is involved. Whereas resistance training may make the interpretation of exercise intensity slightly speculative, many have reported that sprint interval training, such as that included in the CWIT protocol, is sufficiently taxing to the cardiovascular system to elicit positive cardiovascular adaptations (Burgomaster et al., 2005; Gibala et al., 2006; Laursen et al., 2005). The RPE scale is a widely used and accepted method of estimating exercise intensity and gauging an individual’s exercise tolerance (Whaley, 2006). Ratings of perceived exertion have not been reported in previous research with circuit training protocols, yet RPE has been used with other resistance training protocols to monitor exercise intensity (Focht, 2007; Gearhart 2001) and has been shown to be a valid measure (Gearhart 2001). In the current study, subjects perceived the exercise intensity for ACWT and CWIT as higher than that of the TRAD protocol. The RPE measures were higher at the three exercise time points for CWIT than for TRAD (CWIT RPE 38% > TRAD RPE) or ACWT (CWIT RPE 8% > ACWT RPE). Additionally, RPE was also higher at all three exercise time points for the ACWT protocol than the TRAD protocol. The three circuit training protocols in the current study consisted of identical resistance training exercises of similar load volumes. However, the ACWT and CWIT had 15 s rest periods compared to 30 s rest periods in the TRAD protocol. Both ACWT and CWIT protocols had exercise bouts included as part of their respective workouts, with ACWT using aerobic bouts of cycling and CWIT using maximal effort cycling sprints. It is likely that the limited rest and added cycling exercise resulted in the increased RPE measures for CWIT and ACWT. When interpreting RPE results from the current study it should be noted that subjects were asked to provide RPEs upon completion of each mini-circuit station (A1, B2, C3), which in the case of the ACWT and CWIT protocols also included the cycling exercise following each mini-circuit station. This method required subjects to consider all exercises involved in the respective mini-circuit station as well as any exercise that followed. Performing the three familiarization workouts (sessions 2-4) with each protocol prior to the actual data collection sessions gave the subjects an idea of what to expect within each protocol; therefore, the RPE values should be based upon all exercise components within the time period being evaluated as opposed to what exercise was last performed. It is interesting to note that the shape of the HR and RPE curves are relatively similar across TRAD, ACWT, and CWIT protocols with gradual increases observed at each exercise time, albeit at varied extents. The BLA response differed from HR and RPE with concentrations decreasing over time during the TRAD protocol compared to the increases observed with the ACWT and CWIT exercise sessions. As researchers have previously noted, HR may not be the best indicator of cardiovascular load (Petersen et al., 1988), and in order to achieve an adequate VO2 level during a TRAD protocol the workout must be of sufficient duration (Gotshalk et al., 2004). It is likely that when comparing circuit training protocols BLA may serve as the best indicator of exercise intensity. We acknowledge some study limitations. First, although weight exercise volume and intensity were equalized across the TRAD, ACWT, and CWIT protocols, aerobic and interval training components were not equal. A main premise of the study rested on the theory of the inverse relationship between exercise intensity and exercise volume. Each protocol took approximately forty minutes to complete, but exercise intensity was higher during CWIT compared to ACWT and TRAD, respectively. Such unbalanced program comparisons have been made previously to investigate the same principle (Burgomaster et al., 2005; Gibala et al., 2006; Marx et al., 2001). Thus, the three protocols were different in order to compare the amount of fitness-related benefits that can be realized in one workout session, within three different programs of similar duration. Second, the average predicted VO2max of the subjects is low (23 ± 4 ml·kg-1·min-1) for a recreationally active population. Even though subjects participated in familiarization sessions with all testing and training protocols, cycling may have been a relatively unfamiliar testing mode. Also, the use of a submaximal test may have underestimated the predicted VO2max in the recreationally active women from the current study who were not highly trained (Whaley, 2006). Finally, the subjects in this study were recreationally active women who exercised regularly but followed no sport-specific regimen. Thus, results may vary in athletes or others who are involved in specific training activities with consistent neuromuscular demands. CWIT training in a high-lactate environment may have application for athletes from sports that require performing for extended time periods at high intensities (i.e., crew, mixed-martial arts, wrestling). Also, CWIT may be used as an alternative method when athletes have become bored with the daily rigors of regular training. The weights used during CWIT are likely not heavy enough to stimulate sufficient strength gains; therefore, CWIT might be best utilized in a maintenance or pre-season conditioning phase, as opposed to a strength or power phase. Future research is needed to examine the effects of regular training with CWIT protocols. |