Research article - (2024)23, 895 - 906 DOI: https://doi.org/10.52082/jssm.2024.895 |
Acute Neuromuscular Fatigue of a Random Vs Constant Session of Repeated Standing Long Jumps |
Sylvain Dhote1,, Philippe Gimenez1, Sidney Grosprêtre1,2 |
Key words: Random practice, T-reflex, force, countermovement jump, electromyography |
Key Points |
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Experimental approach to the problem |
The experimental design involved a within-subjects’ crossover study, where participants underwent two different jump sessions—one with random intensity (RA) and another with constant intensity (CO)—on separate days in a randomized order. Neuromuscular measurements were taken immediately before and after each session to assess the acute effects of the interventions. Each session lasted approximately 1.5 hours, with 7 days’ rest between sessions. In CO, participants had to perform 100 Standing Long Jumps (SLJ) of constant length. In RA, they had to do 100 SLJ, aiming for different percentages of their maximal jump length at each jump. The maximal horizontal distance (in cm) that each participant could reach was measured during PRE and POST tests and is hereafter referred to as “SLJmax”. In RA, there were 25 SLJ at 65% of SLJ max length, 25 SLJ at 75% of SLJ max, 25 at 85% of SLJ max, and 25 SLJ at 95% of SLJ max. The order in which they did these percentages was randomized. Consequently, the average overall percentage was 80% of max length. In CO, there were 100 SLJ at 80% of SLJ max. In the end, the total distance covered in SLJ was identical in the RA and CO conditions. PRE and POST tests were carried out before and after each bout of repeated SLJs, consisting of different neuromuscular-related measures, as well as effort perception questionnaires. In order to randomize the order in which the percentages of jump length appeared, was used the “RANDOM” formula in Excel software. It uses the Mersenne Twister algorithm (MT19937). |
Participants |
Fifteen participants (13 men and 2 women) were recruited from a sport university, by means of email contacts as well as direct recruitment of acquaintances. They were informed of the benefits and risks of the investigation prior to signing an institutionally approved informed consent document to participate in the study (age: 23.7 ± 3.6 years; height: 174.7 ± 8 cm; weight: 70.8 ± 10.3 Kg; weekly training volume: 8.6 ± 5.3 hours; experience in sport practice: 13.4 ± 4.9 years). They all practiced sports of various kinds for at least 3 years, (street workout, football, gymnastics, amongst others), for a minimum average training volume of 2 h per week. Inclusion criteria were as follows: participants must not have any musculoskeletal disorder; they had to be free of any injury; they were required not to partake of any intense training 48 h prior to the tests; they were required to have at least 6 hours sleep in the night prior to the tests; and were required to abstain from ingestion of any stimulating substances such as caffeine in the 3 h prior to the tests. The study protocol was conducted in accordance with the ethical principles of the Declaration of Helsinki (1983) and approved by the regional ethics committee (Comité d’Ethique pour la Recherche de l’Université Bourgogne Franche-Comté no. CERUBFC-2024-09-03-034). |
Procedures |
The procedure followed by the participants, as well as the various performance tests and neuromuscular measurements, are detailed in the paragraphs below. |
Standing Long Jump (SLJ) |
SLJ consisted in performing a horizontal jump; the starting position was standing still. Jump lengths were measured by a 5-meter Optojump photocell system (Microgate, Bolzano, Italy). This device demonstrated strong concurrent validity and excellent test-retest reliability for the estimation of vertical jump height (Glatthorn et al., The starting position was done with the feet aligned, spaced hip width apart, with the arms straightened in front of the body, and horizontal. Then the movement begins with the hips, knees and ankles bending to create a forward imbalance, as the arms swing behind the trunk. Then the pushing phase begins, with the legs pushing the floor down and behind, and the arms accompanying the movement. During the flying phase, the feet catch up to the body and perform the landing. Participants were asked to jump as far as possible, using a slight forward imbalance and the momentum of their arms. |
Countermovement Jump (CMJ) |
CMJ consisted in doing a vertical jump, with the starting position being standing still. The starting position was with the feet aligned, spaced hip width apart, and the hands placed on the hips. Then, the movement begins with the hips, knees and ankles bending at the same time, until the legs are parallel to the ground, then without interruption, the legs extend, with the goal of reaching the maximal vertical height. The landing is done with the tip of the feet touching the ground first. Flight time was measured by the optojump system at each attempt, and the height of the jump was directly determined by the software from this value. |
Surface Electromyography |
During the whole phase of repeated SLJs, as well as during PRE and POST tests, surface electromyography (sEMG) was recorded. Three wireless electromyographic sensors were placed on 3 knee extensor muscles of the right leg: vastus medialis (VM), vastus lateralis (VL) and rectus femoris (RF) after dry-shaving the skin and cleaning with alcohol. EMG signals from these 3 knee extensor muscles were continuously recorded throughout the experiment. The skin was first shaved and rubbed with alcohol to ensure a low impedance (<5 kΩ). EMG signals were recorded with Trigno sensors (Delsys, Natick, Massachusetts, USA), firmly strapped to the leg with a skin adhesive. Sensors were placed according to SENIAM recommendations (Hermens et al., |
Maximal Voluntary Isometric Contraction |
Determination of maximal voluntary isometric contraction (MViC) in knee extension was carried out before (PRE) and after (POST) the effort of repeated SLJs, on an isometric chair (Leg Control, Mtraining, France). Participants had to perform 2 MViC PRE, and 2 MViC POST, lasting 5 seconds each, with 1 minute rest in between. Continuous verbal encouragement was given during each MViC. Surface EMG of the leg extensor muscles and maximal force production were recorded during MViC. Force signal was continuously recorded at a sampling rate of 2 kHz and stored in Labchart software (LabChart 8, AD Instruments, Sydney, Australia). |
Tendinous reflex |
The deep tendon reflex, or “T reflex”, was assessed by stimulating the patellar ligament with an instrumented reflex hammer (AD Instruments, Sydney, Australia). This hammer is equipped with a force sensor at its tip, which made it possible to know at which force each tap was carried out. Ten tendon taps were performed, inter-spaced by 10 seconds, and with intensities of the strikes randomly varied between 1 and 12 N in order to draw a relationship between tap force and neuromuscular response. The base of the hammer was fixed on a home-made structure in a “pendulum” fashion, so that movement of the hammer head was identical between each tendon strike. All stimulations of the T reflex were done by the experimenter, initiating the pendulum movement manually. |
Testing procedure |
First, participants had to complete a short questionnaire about general information. They had to give their rating on a rating of perception scale, the Borg centiMax scale (CR100), (Borg and Borg, After the warm-up, the PRE-TEST were conducted: participants were tested on their maximal SLJ length and CMJ height. They had to do at least 2 trials for SLJ and CMJ, inter-spaced by 1 min rest. If necessary, more trials were performed until the difference in performance was stable (less than 3% difference). Thereafter, they had to sit on an isometric chair, and T reflex was assessed on their right patellar tendon, with an instrumented reflex hammer as described above. Ten patellar tendon strikes were performed, inter-spaced by 10 seconds, with different strike forces and performed in a random order. Then, they had to perform 2 MViC of the quadriceps muscle of the right leg, lasting 5 seconds, spaced one minute apart. After this PRE-TEST phase, participants did the fatiguing SLJ effort, lasting approximately 20 minutes. The participant had to perform 100 SLJs, successively, with a rest time between each repetition of approximately 10 seconds. In the random (RA) condition, the percentage jump length to be achieved was verbally indicated to the participant before each repetition. Four visual cues were placed on the ground, at percentages adapted to the participant’s maximum performance: 65%, 75%, 85%, 95%. In the constant (CO) condition, a sound signal indicated to the participant to perform the jump, which was always 80%. This effort was performed in 10 sets of 10 repetitions, with 10 seconds of recovery between each set. After this 20-minutes effort, the POST-TESTS were conducted, identically as it was for the PRE tests. Then, participants had to answer a short questionnaire on their perception of the session, consisting of 2 visual analogue scales (VAS) constructed specifically for this experiment, and a questionnaire of mental and physical effort perception (NASA-TLX) (Hart and Staveland, |
Data analysis |
The raw surface EMG data of the knee extensor muscles were processed using the Root Mean Square (RMS) calculated over a moving window of 200 ms in the Labchart -ADinstruments software. The reflex hammer strike force data, as well as leg extension force data were also recorded on this software and synchronized with EMG signals. The reflex stimulation force was obtained with the peak force given by the force transducer in the head of the hammer at each tendon strike. The reflex reaction force of the quadriceps was measured by taking the peak-to-peak amplitude (maximum -minimum) of the force signal. Only the highest of the two MViCs was analyzed. The value for each MViC was taken as the mean value of the force produced over the 5 second contraction. For the evaluation of the deep tendon reflex profile, two different calculations were carried out: 1) The ratio of surface EMG activity in relation to the force of reflex stimulation (= [(peak of the RMS of the EMG signal/maximal amplitude of RMS signal during MViC) / striking force of the patellar tendon * 100], referred to as “RMS/strike”). 2) The ratio of the reaction force to the reflex stimulation force (= [peak force of the quadriceps / striking force of the patellar tendon * 100], referred to as “force/strike”). For each participant, the average of the ratio for each of the 10 tendon stimulations was calculated. All jump length and height data were recorded in the Optojump software, for PRE and POST measurements and for each of the 100 jumps of each session. Mean jump height in cm for each percentage of jump length targeted and for each participant was calculated. The coefficient of variation of the jumping height was calculated for each percentage of jump length targeted, for each participant, as follows: [(standard deviation of jump height in cm / mean jump height in cm) * 100]. The relative error of jump length was calculated for the first 20 SLJs and last 20 SLJs during the repetition of jumps, as follows: [(error of jump length in cm / jump length targeted in cm) * 100]. |
Statistical analysis |
Normality of data and homogeneity of variances were confirmed using the Shapiro-Wilk and Levene tests, respectively. Separate analyses were performed for jump parameters, EMG data, perception of effort, and force production data. Independent samples t-tests were conducted to compare the random vs constant condition, on the Δ of jump performance, on the VAS scores, and on the muscle soreness ratings. Pearson’s correlation was conducted to examine the relation between the patellar ligament strike force and the EMG response, on each participant. One-way repeated measures ANOVA was performed, with the factor Condition (Random and Constant), on the mean jump height in SLJ, and on the CV of the jump height in SLJ. Two-way repeated measures ANOVA was performed, with the factor Condition (Random and Constant) and the factor Time (PRE and POST), on the mean jump height in CMJ, on the mean jump length in SLJ, on the MviCs, on the ratio of Force/strike, on the ratio of EMG/strike, on the relative error of jump length on SLJ, on the 6 items NASA-TLX questionnaire, and on the RPE. For every ANOVA conducted, sphericity of data was confirmed through Maulchy’s test. Greenhouse-Geisser correction was performed when sphericity of data was not verified. The significance threshold was established for a p value <0.05. Both one-way and two-way repeated measures ANOVA were followed by the Bonferroni post hoc test for multiple comparisons. The eta squared statistic is reported as an effect size to describe the magnitude of significant differences (small (η2 = 0.01), medium (η2 = 0.06), and large (η2 = 0.14) effects). Post hoc effect size (ES) was calculated through the standardized mean difference (Cohen’s d) and interpreted as follows: trivial: <0.35; small: 0.35-0.80; moderate: 0.80-1.5; and large effect: >1.5. (Rhea, Statistical analysis was performed using JASP Team software (2022), JASP (Version 0.16.3). |
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Jump performance SLJ |
Two-way repeated measures ANOVA conducted on SLJ length showed no significant effect of time [F (1,14) = 0.251, p = 0.624, η2 = 0.005] but no effect of condition [F(1,14) = 3.101, p = 0.100, η2 = 0.072] and no interaction effect between time and condition [F(1,14) = 1.460, p = 0.247, η2 = 0.028] ( |
CMJ |
CMJ height significantly decreased after both conditions. Two -way repeated measures ANOVA conducted on CMJ height, showed an effect of time [F (1,13) = 21.797, p < 0.001, η2 = 0.626] but no effect of condition [F(1,13) = 0.503, p = 0.491, η2 = 0.012] and no interaction effect between time and condition [F(1,13) = 3.809, p = 0.073, η2 = 0.042] ( |
MVIC |
MViC decreased after both conditions. Two-way repeated measures ANOVA showed an effect of time [F (1,14) = 22.097 p < 0.001, η2 = 0.387] but no effect of condition [F(1,14) = 0.398, p = 0.538, η2 = 0.007] and no interaction effect between time and condition [F(1,14) = 0.808, p = 0.384, η2 = 0.006] ( |
Deep tendon reflex |
Two-way repeated measures ANOVA conducted on the RMS/Strike ratios, showed no significant effect of time [F (1,11) = 3.736 p = 0.079, η2 = 0.043], no effect of condition [F(1,11) = 1.106, p = 0.316, η2 = 0.029] and no interaction effect between time and condition [F(1,11) = 0.408, p = 0.536, η2 = 0.018] ( The ratio of [Force/Strike] decreased overall from Pre to Post. Two-way repeated measures ANOVA conducted showed an effect of time [F (1,13) = 5.735 p = 0.032, η2 = 0.206] but no effect of condition [F(1,13) = 0.938, p = 0.351, η2 = 0.014] and no interaction effect between time and condition [F(1,13) = 0.491, p = 0.496, η2 = 0.004] ( EMG during MViC significantly decreased overall from Pre to Post. Two-way repeated measures ANOVA showed a significant effect of time [F (1,14) = 9.991 p = 0.007, η2 = 0.160], no effect of condition [F(1,14) = 0.036, p = 0.852, η2 = 0.001] and no interaction effect between time and condition [F(1,14) = 0.054, p = 0.820, η2 < 0.001] ( As shown in |
Jump variability |
Jump height during SLJ increased with increasing targeted length. One-way repeated measures ANOVA showed a significant effect of the relative jump length on the jump height adopted by the participants [F (1.538; 42) = 82.903, p < 0.001, η2 = 0.856] ( The CV of the jump height decreased with increasing targeted length. One-way repeated measures ANOVA showed a significant effect of the relative jump length on the coefficient of variation of the jump height [F (4; 52) = 7.262, p < 0.001, η2 = 0.358] ( Two-way repeated measures ANOVA carried out on the relative jump length errors showed no significant effect of condition [F (1,13) = 0.018, p = 0.896, η2 < 0.001], no effect of time [F (1.13) = 1.455, p = 0.251, η2 = 0.030], and no interaction effect between time and condition [F (1,13) = 0.783, p = 0.393, η2 < 0.028] ( |
Perceptual results |
RPE increased only after the RA effort. Two-way repeated measures ANOVA carried out showed a significant effect of time [F (1.13) = 6.924, p = 0.021, η2 = 0.225], but no significant effect of condition [F (1,13) =0.137, p = 0.717, η2 = 0.002], and no significant interaction between time and condition [F (1,13) = 2.073, p = 0.174, η2 = 0.020] ( On the visual analogue scale (VAS) n°2 (measuring the perceived difficulty of the required precision), the T test did not show a significant difference between CO and AL. On VAS n°2; (measuring the boring/amusing nature of the activity), the T test showed that the RA condition was perceived to be more entertaining than the CO condition (p = 0.003) ( Concerning the NASA-TLX test, the T tests only showed a difference between the conditions for question no. 6 (“How successfully do you think you carried out this work?”), the participants having perceived greater success in the RA condition (p = 0.032). The first question (“What degree of mental and/or perceptible activity was required?”) was rated higher in the RA condition, but it was a nonsignificant difference. (p = 0.069) ( The paired sample T-tests performed on the peak muscle soreness ratings (DOMS) revealed no significant differences between conditions, regardless of the muscle group. |
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The aim of this study was to determine whether randomizing the intensity of a repeated motor task would have an impact on physical performance and on neuromuscular and perceived fatigue, as compared to a more traditional approach with fixed intensity. The results of this study show that acute sessions of random (RA) practice and constant (CO) practice have similar effects in terms of physical fatigue and neuromuscular system behavior. However, RA appeared to benefit participants mentally, as greater enjoyment was reported during random sessions. |
Physical performance |
First, the performance of participants on the CMJ max test decreased significantly overall, regardless of the condition. Our goal was to propose a jumping session that would be fatiguing enough to decrease the performance of participants on this particular test, which we believe mostly reflects the neuromuscular and peripheral fatigue. In the present experiment, on average, considering both conditions, jump height in CMJ decreased by 5%. This result is similar to a study (Raeder et al., Secondly, the performance decrease occurring in POST did not differ between the CO and RA conditions in CMJ max, SLJ max, or MViC of the leg extension. In order to examine the impact of the organization of the exercise session alone, the training load was equalized in the random and constant conditions. Thus, we can speculate that with matched training loads, the design of the fatiguing session does not affect the muscular fatigue induced, even if this hypothesis can be challenged by results of previous studies. One investigation (Gorostiaga et al., Nevertheless, there was a difference in the decrease in performance between the 2 maximal jumps assessed in PRE and POST: there was a significant decrease in the maximal height reached in CMJ, resulting from the fatigue induced by repeated jumps, but no significant decrease in the maximal jump length reached in SLJ. Similarly, another study (Watkins et al., |
The neuromuscular system |
Surface EMG data was recorded during MViC to detect whether the neuromuscular factor would be responsible for the fatigue induced. Indeed, according to a recent meta-analysis (Hou et al., We also sought to verify whether the induced fatigue would be more pronounced after a random session, as the neuromuscular demand would be greater when the participants are exposed to greater variability. It turns out that there is indeed a decrease in peak RMS of EMG during the MViCs after the repeated jumps, but it is not different after the random condition. This suggests that central fatigue occurred because of the repeated jumps, regardless of the condition, and possibly originating from spinal and/or supraspinal levels (Gandevia, Results on the T-reflex test showed a possible involvement of spinal adaptation to the observed fatigue. Indeed, a decrease in the ratio of [reflex force/strike force] could suggest decreased spinal excitability with fatigue. However, no significant change in the [RMS/strike force] ratio was found (p = 0.079). The absence of interaction effect signifies that the decrease in reflex intensity was similar after constant and random effort. As the random intensity of effort during repeated SLJ does not imply greater fatigue of the spinal reflex, this questions a previous hypothesis on the effect of random practice on the neuromuscular system. Either the present results are incomplete and were unable to reveal the actual effects of random practice, or the random intensity of effort is no different to constant intensity in terms of acute fatigue of the neuromuscular system. Deeper investigations of the spinal loops after random exercises are warranted at this stage, using H-reflex techniques for instance. |
Variations in performance |
It has been demonstrated that random practice is beneficial for performance in the long term, i.e. as assessed with retention tests, in the context of basketball (Porter and Magill, The coefficient of variation of the jump height (CV) was significantly lower for the 95% SLJ distance than in 65%, 75% and 80% (p<0.05). This result can be interpreted as a change in the jumping strategy, which depends on the percentage of SLJ distance to cover. The study by Grosprêtre et al. in 2018 showed that expert parkour practitioners were able to jump further than beginners on the SLJ, and that for higher percentages of maximal distance, they adopted a jumping strategy where they slightly diminished the jump height (by a decrease of the angle of take-off), thus increasing the jumping length. Consequently, the CV of the jump height necessarily decreases, as there are fewer options in the angle at take-off to reach the near maximal SLJ distances. |
Subjective markers of fatigue |
Overall, results related to perception of fatigue showed that there were differences between the conditions, and between pre and post effort. Firstly, the CR100 (Borg) scale results showed that the general state of subjective fatigue increased significantly only after the random effort. Although randomization of the jumping session may be responsible for a greater state of fatigue at the end, this higher rating could also be due to the increased mental demand in the random condition, as the question they were asked was: “what is your general state of fatigue right now”, including both physical and mental fatigue. Interestingly, the physical demand seems to be identical, based on the various performance tests carried out. Thus, the greater mental demand of the random condition could be attributed to the near maximal jumping percentages, especially the 95% maximal jumping length. Indeed, even though, on average, the jumping length to achieve was 80% overall in the constant and in random conditions, that does not necessarily mean that average intensities were identical. Perhaps there is a nonlinear relation between the percentage of jump length and the actual intensity required. This hypothesis is supported by the results of a similar study (Grosprêtre et al., In a meta-analysis (Habay et al., The better appreciation of the random condition compared to the blocked condition, as shown by the differences in ratings on the NASA-TLX, and the adapted VAS, is in line with previous research on the effect of variability on the rating of perceived exertion (Streder, From a more general point of view, random practice, when compared to blocked practice with similar training load, seems to produce very similar physical stress, but is concomitantly better appreciated by participants. Thus, it is an interesting result, as this could encourage sports practitioners to implement this kind of training organization, especially during a phase where the monotony of training would be high. The present study has some limitations. Firstly, there was no comparison of a variable and organized session to a variable and non-organized session. Thus, the effect of the random condition in the present study, which can be considered as variable and non-organized, cannot be attributed solely to one of these two components (variability or disorganization) but rather, only to both of them together. Moreover, it would be of interest to consider motor strategy adaptations throughout the 100 jumps, by means of kinematic analysis. Indeed, a different use of the arm swing, for instance, could interfere with jumping performance, and could be quantified by means of motion capture. |
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In conclusion, the results of the present experiment suggest that between random practice and blocked practice, the differences lie mostly in the perceptual aspect. The physical performance, as assessed by maximal strength testing, as well as the jump height and length, do not show any significant differences. Interestingly, very little changes in terms of the movement variation (only the jump length from 65 to 95% of maximum) leads to markedly higher practice enjoyment. Specifically, within a given, monotonous session of 100 repeated and identical standing long jumps, randomization of the practice design seems to make the session clearly more tolerable and more entertaining for the participants. The training load calculation was simple, in order to be as applicable to the field as possible and was chosen basically as the sum of all the distances covered over the 100 repeated SLJs. According to our results, coaches may utilize this simple method to implement random sessions of exercise instead of the traditional sessions, with equivalent training load. The benefit of this particular random design is to be more positively perceived by athletes, and it could be implemented during monotonous phases of training, with the intent on boosting the progression. Future research should investigate the effect of random and regularly varied intensities of jumps, for example, by intermittent training. More generally, there is a need to examine the effects of a randomly organized intensity of effort during specific movement associated with a sporting discipline. Researchers should also be aware of the clear distinction between effort of randomized intensity, established in advance, and effort with unpredicted intensity, because chosen by the participants or the athletes, and must design future research accordingly. |
ACKNOWLEDGEMENTS |
The experiments comply with the current laws of the country in which they were performed. The authors have no conflict of interest to declare. The datasets generated and analyzed during the current study are not publicly available but are available from the corresponding author upon reasonable request, who was an organizer of the study. |
AUTHOR BIOGRAPHY |
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REFERENCES |
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