Table 1. PCR primers for MMPs, TIMPs, and GAPDH
gene sense and antisense primers cDNA size (bp)
MMP-1 5†²-CACAGCTTTCCTCCACTGCTGCTGC-3†²
5†²-GGCATGGTCCACATCTGCTCTTGGC-3†²
396
MMP-2 5’-GACAAGAACCAGATCAATAC-3’
5’-GCCATGCTCCCAGCGGCCAAA-3’
181
MMP-13 5†²-TGGTGGTGATGAAGATGAT TTGTCT-3†²
5†²-AGTTACATCGGACCAAACTTTGAAG-3†²
366
TIMP-1 5†²-CCTGGCTTCTGGCATCCTGTT-3†²
5†²-GGGACCTGTGGAAGTATCCG C-3†²
280
TIMP-2 5†²-CAGTGAGAAGGAAGTGGACTC-3†²
5†²-CATCTGGTACCTGTGGTTCAG-3†²
300
TIMP-3 5’-ACGCCTTCTGCAACTCCGACA-3’
5’-CCTCTCAGCAGGTACTGGTAC-3’
221
GAPDH 5†²-CCACCCATGGCAAATTCCA TGGCA-3†²
5†²-TCTAGACGGCAGGTCAGGTCCACC-3†²
600