Table 1. Characteristics of the included studies.
Study Participants Characteristics Stretching method Muscles stretched Amplitude/intensity Volume (sets, duration/repititions) Velocity/frequency Flexibility outcome
Barbosa et al. (2018) Healthy, physically active males Total: n = 45,
DS: n = 15 (21.47 ± 3.0 years),
CG: n = 15 (21.27 ± 2.8 years)
DS Hamstrings Mild discomfort 30 reps × 3 sets Established “beep” rate Knee extension ROM
Behm et al. (2011) Young and middle-aged males Total: n = 18,
Young: n = 10 (22 ± 1.4 years),
Middle-aged: n = 8 (46.3 ± 6.5 years)
DS Quadriceps,
Hamstrings,
Plantar flexors
The highest ROM possible for all DS 8 reps (30 s) × 3 stretches Not reported Hip extension ROM,
Sit-and-reach test
Chen et al. (2018) Healthy young males Total: n = 36 (22.0 ± 1.5 years),
DS (CKC): n = 12,
DS (OKC): n = 12,
CG: n = 12
DS (CKC),
DS (OKC)
Hamstrings ROM (less than the point of discomfort) DS (CKC): 8 reps (15 s) × 6 sets,
DS (OKC): 15 s × 6 sets
DS (CKC): 1:1 s,
DS (OKC): Not reported
Straight-leg raise test
Hernández-Trujillo et al. (2024) Healthy, recreationally active males and females Total: n = 60 (46.4 ± 5.5 years, 30 males and 30 females),
BS: n = 20 (10 males and 10 females),
CG: n = 20 (10 males and 10 females)
BS Quadriceps,
Hamstrings,
Hip extensors,
Hip flexors,
Hip adductors,
Hip abductors
ROM 20 s × 3 sets × 6 stretches Following the metronome rhythm (40 bpm) Hip flexion ROM,
Knee flexion ROM
Inoue and Nagano (2023) Healthy adult males Total: n = 12 (21.2 ± 0.6 years) DS, BS Hamstrings 70%–90% of the maximum value of the POD 2 min × 4 sets Not reported Straight-leg raise test
Jung et al. (2023) Healthy young males and females Total: n = 44 (36 males and 8 females)
DS: n = 11 (26.27 ± 1.68 years, 9 males and 2 females),
BS: n = 11 (26.73 ± 2.15 years, 9 males and 2 females),
CG: n = 11 (27.45 ± 3.17 years, 9 males and 2 females)
DS, BS Plantar flexors DS: Not reported,
BS: 2–5° at the end ROM
45 s × 4 sets DS: Once per second,
BS: Twice per second
Ankle DF ROM
Kaneda et al. (2020) Healthy young males Total: n = 17 (23.2 ± 1.1 years) DS Hamstrings ROM 15 reps (30 s) × 4 sets Once every 2 s Straight-leg raise test,
Passive knee extension test
Konrad et al. (2017) Healthy young males and females Total: n = 122 (79 males and 43 females),
BS: n = 24 (22.6 ± 2.8 years, 16 males and 8 females),
CG: n = 24 (23.8 ± 3.5 years, 11 males and 13 females)
BS Planter flexors The last 5° of the individuals’ ROM 30 s × 4 sets At a frequency of 1 Hz Ankle DF ROM
Mizuno (2017) Healthy young males and females Total: n = 15 (8 males and 7 females, 23 ± 2 years) DS (1 set),
DS (4 sets),
DS (7 sets)
Plantar flexors ROM 15 reps (30 s) × 1 set,
15 reps (30 s) × 4 sets,
15 reps (30 s) × 7 sets
Once every 2 s Ankle DF ROM
Mizuno and Uemura (2016) Healthy young males and females Total: n = 12 (8 males and 4 females, 23 ± 1 years) DS Plantar flexors ROM 15 reps (30 s) × 4 sets Once every 2 s Ankle DF ROM
Muanjai and Namsawang (2023) Healthy young and older adult females Total: n = 30,
Young: n = 15 (20.9 ± 0.7 years),
Older: n = 15 (66.6 ± 4.2 years)
DS Hamstrings The point of maximum discomfort without pain 8 reps × 6 sets Stretch the hamstring muscles for 1 s and 1 s down back to the starting position Straight-leg raise test,
Sit-and-reach test
Murphy et al. (2010) Healthy, physically active males Total: n = 42,
DS: n = 13 (20 ± 2 years),
CG: n = 15 (21 ± 2 years)
DS Pectoralis major,
Deltoids,
Latissimus dorsi,
Hamstrings,
Quadriceps,
Gluteus maximus,
Hip flexors, Calves
Until subjects felt slight discomfort, but not to a range of motion that produced pain 10 reps (20 s) × 12 stretches Once every 2 s Hip flexion ROM,
Knee flexion ROM,
Sit-and-reach test
Nelson and Kokkonen (2001) College students Total: n = 22 (males: 23.3 ± 2.7 years, females: 21.6 ± 3.0 years, 11 males and 11 females) BS Quadriceps,
Hamstrings,
Gluteals,
Hip Adductors,
Plantar flexors
2–5° at the end of the ROM 15 s × 3 sets unassisted × 5 stretches,
15 s × 3 sets assisted × 5 stretches
Once per second Sit-and-reach test
Oliveira et al. (2018) Trained young male soccer players Total: n = 12 (17.67 ± 0.87 years) BS Quadriceps,
Hamstrings,
Hip extensors,
Planter flexors
The maximum POD 30 s × 3 sets × 4 stretches 1:1 s Sit-and-reach test
Paradisis et al. (2014) Active adolescent boys and girls Total: n = 47 (14.6 ± 1.7 years, 17 males and 30 females) DS Quadriceps,
Hamstrings,
Hip extensors,
Plantar flexors
Not reported 20 reps (40 s) × 4 stretches Once every 2 s Sit-and-reach test
Ryan et al. (2014) Healthy males Total: n = 26 (22.2 ± 1.3 years) DS,
DS (twice the volume)
Quadriceps,
Hamstrings,
Hip extensors,
Hip flexors,
Hip adductors,
Hip abductors,
Plantar flexors
ROM DS: 4 reps × 8 stretches, 6 reps × 3 stretches,
DS (twice the volume): 8 reps × 8 stretches, 12 reps × 3 stretches
In a controlled manner through a ROM Sit-and-reach test
Wiemann and Hahn (1997) Healthy males Total: n = 69 (aged 20 to 34),
BS: n = 16,
CG: n =15
BS Hamstrings Not reported 15 s × 3 sets × 3 stretches Rhythmically Hip flexion ROM
DS: dynamic stretching, BS: ballistic stretching, CKC: closed kinetic chain, OKC: open kinetic chain, CG: control group, ROM: range of motion, POD: point of discomfort, DF: dorsiflexion. Values are expressed as the mean ± standard deviation.