This is a cross-sectional baseline study, which is part of a school-based intervention PA program carried out in 5 primary and 2 middle-high public schools from a suburban setting from Oporto-Portugal. Over a period of 6 months, 159 students volunteered to attend a PA program, twice a week. For this cross-sectional study, the sample was comprised by 131 children (83 girls, 7-15 year-olds) involved in the program who: (1) did all the baseline testing procedures; (2) were not attending any other formal sports or PA program; and (3) were classified as obese, according to age-gender specific body mass index (BMI) cut-off points (Cole et al., 2000).

For the purpose of this study, the sample size was calculated using the G*Power software 3.1.9.2. Hypothesizing an effect size (Cohen’s d) of 1.0 for a required power of 95% at p<0.05, a sample size of at least thirty subjects was required. Therefore, a larger sample size of 131 subjects should provide adequate statistical power for the analysis.

The Regional Education Board approved the study protocol, and students, parents and schools agreed to participate. The nature, benefits, and risks of the study were explained to the volunteers, and a parent’s written informed consent was obtained before the study, consistent with the Helsinki Declaration. The Scientific Board of the Research Unit from The Faculty of Sports of Oporto University approved the evaluation methods and procedures used.

One week before the evaluation week, all the subjects were familiarized with the testing protocols, procedures and equipments. Six Physical Education teachers, three pediatric nurses, and a medical doctor carried out all measures, which took three consecutive mornings. Participants were identified through an individual code number and a school code number, and divided in three groups, according to their convenience. One group was evaluated per day. Fasting blood samples were taken between 08:00am and 9:00am. The children and adolescents were then given breakfast, followed by the evaluation of the anthropometric, body composition, maturational, and cardiorespiratory fitness variables, between 09:30 am and 11:00 am. At the end of the evaluation protocol, the accelerometers were given to the students. Participants’ parents were recommended to encourage their children to maintain the same standard meal.

Body height was measured to the nearest mm in bare or stocking feet with the adolescent standing upright against a Holtain Stadiometer. Weight was measured to the nearest 0.1 kg, lightly dressed and after having breakfast, using an electronic weight scale (Seca 708 portable digital beam scale). BMI was calculated from the ratio of body weight (kg) / body height (m²). Waist circumference (WC) was evaluated using the NHANES protocol (Kuczmarski, 1996). A bony landmark is first located and marked. The examiner, positioned to the right of the participant, palpated the upper hip bone to locate the right iliac crest. Just above the uppermost lateral border of the right iliac crest, a horizontal mark was drawn, and then crossed with a vertical mark on the mid-axillary line. The measuring tape was placed in a horizontal plane around the abdomen at the level of this marked pointed on the right side of the trunk. The plane of the tape was parallel to the floor and the tape was held snug, but did not compress the skin. The measurement was made at a normal minimal respiration.

Blood samples were collected by three pediatric nurses. A mean inter-observers and intra-observers coefficient of variation (CV) of 2.4 and 1.2% respectively, was observed.

Blood was collected from the antecubital vein between 8:00 and 09:00 a.m., after at least ten hours of fasting. Blood samples were obtained on a fasting basis and processed within 2h of collection. Blood was obtained by venipuncture, to EDTA containing tubes. Following centrifugation, plasma was separated, aliquot and immediately stored at -80°C until the end of the week, when it was assayed. To determine ALT concentrations, a routine automated technology (ABX Diagnostics) was used. The biochemical evaluation of all participants was conducted in the same laboratory.

PA was objectively assessed by accelerometers (wGT3x, Actigraph, Florida) during 7 consecutive days. Data was stored in raw mode in samplings of 30Hz. With a specific software (ActiLife, version 6.9, Actigraph, Florida), data was reduced into one-minute periods (epochs), organized into daily physical activity and analyzed after data collection. Wear and nonwear time was determined according to Choi et al. (2011) algorithm. Time periods with at least 10 consecutive minutes of zero counts recorded were excluded from analysis assuming that the monitor was not worn. A minimum recording of 8-hours/day (480-minutes/day) was the criteria to accept daily PA data as valid. Individual’s data were only accepted for analysis if at least three-week days and one weekend day were successfully assessed. The main outcomes of reduced accelerometer data were: total physical activity [total PA (counts/min/day)], time in sedentary behavior [SB (min/day)], light physical activity [LPA (min/day)], moderate physical activity [MPA (min/day)], vigorous physical activity [VPA (min/day)] and moderate to vigorous physical activity [MVPA (min/day)].

International recommendations (Trost et al., 2011, Evenson et al., 2008b) cut-points were used to determine time spent in PA of different intensities. The following counts intervals were considered: 0-100 for SB, 101-2295 for LPA, 2296-4011 for MPA, and ≥4012 for VPA (Evenson et al., 2008a)For the analysis we used MVPA, defined as the sum of MPA and VPA.

To assess the maximal oxygen uptake (VO_2max), all subjects were submitted to a continuous progressive treadmill exercise. Participants were instructed to walk/run until exhaustion, according to a standardized exercise protocol (Eiberg et al., 2005a; 2005b). The exercise protocol started at 4 km·h^-1 without inclination and maintained for 3 minutes so that subjects could adapt to the treadmill. After 3 min, speed was increased to 8 km·h^-1. When 5 min were completed, the inclination was raised to 3%. After 7 and 9 min, the inclination was increased to 6% and 9%, respectively. If the subjects were able to endure more, the speed was increased to 9 km·h^-1 after 1 min and then 10 km·h^-1 after 13 min.

Oxygen uptake was measured directly with Oxycon^TM Pro Metabolic Cart (Jaeger^TM, Höchberg, Germany). Respiratory variables were recorded breath-by-breath, which in turn were averaged over a 5-seconds periods, yielding a “fair” representation of the change in VO₂ during incremental exercise (Astorino, 2009). Before each individual test, oxygen and carbon dioxide analyzers were calibrated according to the manufacturer’s instructions. Directly measured VO_2max or peak VO₂ was the main variable determined using the open-circuit method. Directly measured VO_2max was considered when a plateau in the VO₂ curve was detected, defined as an increase in a VO₂ of less than 2 ml^.kg^-1.min^-1 with a concomitant increase in speed stage. If a VO₂ plateau was absent (Docherty, 1996), the peak VO₂ was taken and defined as the highest oxygen uptake achieved during exercise at exhaustion (Armstrong and Welsman, 2007). For practical reasons, from now on, this paper will refer to the highest VO₂ values achieved as VO₂max. Exhaustion was confirmed when: (1) subjects desired to stop or demonstrated an inability to maintain the required running pace despite strong verbal encouragement; (2) maximal heart rate was greater than 85% of age-predicted maximal heart rate (220-age); (3) the respiratory exchange ratio (RER) was greater than 1.0 at the end of the test; (4) the participants showed symptoms of discomfort and/or signs of high sweating, facial flushing and grimacing (Docherty, 1996).

Careful control was taken concerning technical and environmental variables that might have had some influence on the results, so that highly reliable metabolic measures could be obtained.

Regional (arms, legs, trunk, and head) body fat mass was measured using a whole-body DEXA scanner (Hologic QDR-2000, software version V5.67A, Hologic Inc., Bedford, MA). The standard procedures described in the literature for DEXA measurements were employed (Paradisi et al., 1999, Wong et al., 2002). The coefficient of variation of the instrument was < 1% for in vivo measurements. The body regions were delineated by means of specific anatomical landmarks, and central fat mass was calculated as the trunk fat mass.

Maturational stage was determined on an individual basis during physical examination. Each subject self-assessed his/her own stages of secondary sex characteristics: stage of breast development in females and pubic hair in males (Tanner, 1962). A previous study showed a high correlation (r=0.73) between ratings on two occasions (three day interval) in a sub-sample of 50 selected subjects. Concordance between self-assessments of sexual maturity status and physician assessment ranged from 63% for girls and 89% for boys (Mota et al., 2002). This information was used as a co-variable in the statistical procedures.

All variables were checked for normality and homogeneity, and appropriately transformed when necessary. Descriptive statistics (mean and range values) were used in order to characterize the participants.

Independent sample t-test was used to analyze differences between genders for all the measured variables. To analyze how variables correlate, three different models for Partial Correlations were calculated. The first one analyzed the association between ALT with different intensities of PA, central adiposity, and CRF, adjusted for potential confounders (gender and maturational stage). In the second model, we adjusted the analyses for central obesity. In the third one we added a further adjustment for CRF. The associations between ALT with PA intensities, central obesity, and CRF were calculated by linear regression and followed the same three models used for calculating the partial correlations.

Analyzes were performed with the statistical software package (SPSS 20.0 for Macintosh) and level of significance was set at p ≤ 0.05.