In the current study, the running pattern of the lower extremity was examined while being perturbed through tubes attached between the ankles and the lower back to analyze influences on the running pattern variability before and after a varied running intervention. 3D-kinematics, joint coupling and electromyography (EMG), as well as their variability, were analyzed in ten healthy male participants during treadmill running (10.5 km·h^-1). Pre- and post-tests each consisted of 2 x 30 min treadmill running (one with and one without tubes). The results showed major acute effects on EMG and kinematics, as well as joint coordination variability, due to the constraints (p < 0.05). After the intervention, a process of normalization of most kinematic and EMG parameters occurred; however, EMG variability, kinematic variability and joint coordination variability were reduced during tube running below normal running level (p < 0.05). The findings further indicate rapid kinematic adaptations while muscle activity appears to require longer practice to adapt. The constraint serves to acutely increase variability, but may lead to reduced variability when applied for a longer period of time.