This study aims to identify the stroke mechanics regulations that underlie pacing optimization and differentiate final performance in the 400 m front-crawl across different performance levels. 157 all-out 400 m trials from trained to elite swimmers equipped with a sacrum worn IMU were analyzed. Hierarchical generalized additive models were fitted to describe (1) the technical predictors of final performance, through the interrelationships between averaged kinematics (i.e., stroke rate (SR), stroke length (SL), jerk cost (JC)) and mean speed, and (2), the profiles of pacing and associated stroke mechanics regulations leading to performance. These models depict both the common patterns shared by all swimmers and the specific group deviations by performance level (G1 -slowest- to G4 -fastest-) from population trends. All swimmers shared a reverse-J-shaped pacing (p < 0.001). The fastest swimmers reduce pacing variability with a relatively slower start, a minimized drop in speed in the middle of the race, and a greater end-spurt than the population trend (p < 0.05). Their stroke mechanics are effectively adapted to the requirements of such specific race sections, both through a U-shaped JC with higher magnitude (p < 0.001) and a continuous adaptation of the SR (p < 0.01) and SL (p < 0.01) combination. A high average SL (i.e., at least 1.50 m.stroke^-1), coupled with an SR close to 70 strokes.min^-1 and maximizing stroke smoothness at race pace, are critical technical abilities for optimizing final performance. Technical training should prioritize the reduction of intra-cyclic acceleration variations at race pace as a key driver of 400 m performance development. The best performers lower pacing variability by amplifying the magnitude of kinematic regulations, particularly in terms of stroke smoothness management. Such underlying adaptations in stroke mechanics play a pivotal role in continuous speed control throughout the race. The findings of this study enhance the technical understanding of discriminative 400 m pacing skills for coaches and swimmers.

• Technical understanding of distinguishing pacing skills in the 400 m freestyle is enhanced by highlighting the associated regulations of stroke mechanics, providing insights for performance development.

• Pacing strategy and final performance are underpinned by effective stroke smoothness management and a continuous adaptation of the stroke rate (SR)-stroke length (SL) combination, tailored to the demands of specific race sections.

• An average SL of 1.50 m.stroke coupled with an SR close to 70 strokes.min and maximizing stroke smoothness at race pace are critical kinematic abilities.